Presentation Profile
Advancements in high matrix neutralization for sample preparation of surface, ground, and wastewater samples.
Currently Scheduled: 10/15/2025 - 12:30 PM - 12:50 PM
Room: South Lobby
Main Author
Christopher Mitchell - Biotage
- Evan Walters - Biotage
- Deanna Bissonnette - Biotage
Abstract:
Environmental laboratories routinely follow the Environmental Protection Agency (EPA) 1600, 600, and 8000 series methods to analyze contaminants in surface water, groundwater, and wastewater. However, the physical and chemical complexity of these samples present significant challenges during sample preparation, including high particulate loads and matrix interferences. To address these challenges, many laboratories have adopted methods to reduce sample preparation volumes from the traditional 1L samples. While this approach minimizes handling difficulties, it can negatively impact laboratory reporting limits by increasing the detection limits for target analytes. To overcome these limitations, we introduce a novel 47mm depth filtration disk holder incorporating the ISOLUTE® HM-N filtration product. This system effectively neutralizes high-matrix interferences and integrates seamlessly with solid-phase extraction (SPE) techniques. Laboratories can process 1L wastewater samples in under 10 minutes with unprecedented ease and yield a 35% reduction in solvent use. This innovative sample matrix filtration approach enhances performance criteria by reducing detection limits, increasing accuracy, and improving precision when compared to traditional liquid-liquid extraction and micro-extraction techniques. This work highlights the transformative potential of this new technology to simplify and optimize sample preparation workflows. Environmental laboratories that implement the 47mm depth filtration disk holder for EPA 1600, 600, and 8000 series methods will maximize sample processing efficiency, improve sustainability, and produce reliable data.
