Presentation Profile
Analysis of DNPH-derivatized Aldehydes and Ketones using Agilent iQ Single Quadrapole LC MS
Currently Scheduled: 10/11/2023 - 9:30 AM - 9:50 AM
Main Author
Sue Dantonio - Agilent
- Robert Dantonio - Texas A and M Corpus Christi
- Nikolas Lau - Agilent Technologies
- John Wright - Agilent Technologies
Abstract:
Aldehydes and ketones are important compounds in the chemical industry. However, these compounds can be hazardous when released into the environment. Many chemical plants, industrial hygienists and air monitoring agencies preform quantitative analysis on air samples. Typically, this analysis is done by HPLC with DAD detection as all of the compounds have excellent chromophores after derivatization with 310 being the typical uv wavelength. This method works very well, When an unknown peak appears in a run, that peak needs to be identified as a hazardous or non-hazardous compound. Therefore is this set of experiments, our goal was to lower the LOD and LOQ, and use see interferences and unidentified peaks in real world samples. Methods In this set of experiments, we ran n equals 3 replicates of standard, TO11/IP-6A Aldehyde/Ketone-DNPH Mix from 1 ppb to 1000 ppm to create large calibration curves. The chromatography removed the acetone for the solvent Acetonitrile and added formic acid to add ionization. We also were given an air filter sample cartridge. This cartridge was extracted with pure acetonitrile and run on a C18 column. A diode array detector was run in series with the LC MS. Samples and standards were run with both SIM and Scan mode. We were able to create a library with known compounds of interference. We were able to both identify interfering compounds, and to quantitate in the same analytical run.
