Presentation Profile
Comprehensive two-dimensional gas chromatography with Orbitrap mass spectrometry applied to flavor and fragrance analysis
Currently Scheduled: 10/15/2019 - 10:30 AM - 10:50 AM
Room: South Lobby
Main Author
Xin Zheng - Thermo Fisher Scientific
- Jason Cole - Thermo Fisher Scientific
- Matthew Edwards - SepSolve
Abstract:
High Resolution/Accurate Mass (HRAM) mass spectrometry (MS) has become a popular detector for GCxGC as it provides full-scan analysis with high-quality mass spectra and excellent sensitivity and selectivity, especially for trace level detections. Orbitrap mass spectrometry is superior to detect low concentration unknowns and elucidate their chemical formula with sub ppm mass accuracy and extremely wide dynamic range, which dramatically increases confident identification. In this study, an HRAM Orbitrap analyser was coupled with a GCxGC using reversed flow modulation for flavor and fragrance analysis. Different subtypes of lavender oils were analyzed. The complex matrices and coelution are significant challenges for one-dimensional (1D) GC-MS. Diverse structurally similar isomers can be still problematic even HRAM mass spectrometry is applied due to the exact same masses for both molecular and fragment ions. The flow-modulated GCxGC-Orbitrap MS can effectively address this issue and separate multiple chemical classes such as monoterpenes, sesquiterpenes and oxygenated terpenes that co-eluted in one-dimensional separation. Excellent mass accuracy over a wide dynamic range was achieved as well.
