Presentation Profile
Optimizing the Interface between Supercritical Fluid Chromatography and Electrospray Ionization Mass Spectrometry
Currently Scheduled: 1/18/2018 - 2:00 PM - 2:20 PM
Room: Room 381 A
Main Author
Michael O. Fogwill - Waters Corporation
Abstract:
Supercritical fluid chromatography (SFC) employs a compressible carbon dioxide-based mobile phase. Since the solvating power of the SFC mobile phase is directly related to pressure, there is a dramatic difference in mobile phase properties from the point of sample introduction to the point of detection in a SFC system coupled with mass spectrometry (MS). In order to maintain excellent peak shape and maximize sensitivity, both instrument design and method development must be optimized to account for this change in mobile phase solvating power. When properly optimized, SFC-MS provides a very sensitive and robust interface with performance on par with liquid chromatography mass spectrometry. This presentation will outline optimization of the instrumentation and the tuning parameters for the SFC-MS interface. Troubleshooting techniques specific to SFC-MS will be discussed with application examples.
