Presentation Profile

Identification and Discrimination of Bacteria by Gas Chromatography – Vacuum Ultraviolet Spectroscopy Analysis of Fatty Acids

Currently Scheduled: 1/18/2018 - 2:30 PM - 3:00 PM
Room: Room 381 B

Main Author
Ines Santos Post-Doc - The University of Texas at Arlington

Abstract Number: 234
Abstract:

The use of bacterial components such as fatty acids has increasingly been applied for the taxonomic classification of microorganisms as these profiles are unique to each species, and the methods are fast and reliable. This information is mainly obtained by gas chromatography (GC) coupled with mass spectrometry; however, the position and geometry of the double bonds are difficult to elucidate. As an alternative, a new, universal vacuum ultraviolet (VUV) detector has been developed that as the ability to distinguish isomers. In this work the bacterial fatty acid methyl esters were analyzed by GC-VUV and used to identify and discriminate environmental microorganisms.

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